Stability of Silica-Based, Monofunctional C18 Bonded-Phase Column Packing for HPLC at High pH
نویسندگان
چکیده
منابع مشابه
Physical origin of peak tailing on C18-bonded silica in reversed-phase liquid chromatography.
Single component isotherm data of caffeine and phenol were acquired on two different stationary phases for RPLC, using a methanol/water solution (25%, v/v, methanol) as the mobile phase. The columns were the non-endcapped Waters Resolve-C18, and the Waters XTerra MS C18. Both columns exhibit similar C18 -chain densities (2.45 and 2.50 micromol/m2) and differ essentially by the nature of the und...
متن کاملSurface Characterization of Some Novel Bonded Phase Packing Materials for HPLC Columns Using MAS-NMR Spectroscopy
Information on the surface properties of three novel chemically bonded phase packing materials for High performance liquid chromatography (HPLC) were obtained using spectra obtained by solid state cross-polarization (CP) magic-angle spinning (MAS) nuclear magnetic resonance (NMR) spectroscopic experiments for the 29Si, and 13C nuclei. These packing materials were: Cogent bidentate C18 bonded to...
متن کاملApplication of Monolithic Silica Column for HPLC Separation of Estrogens
A monolithic silica column has recently been widely used for HPLC separation analysis. Compared with the particle−packed column (conventional column), the monolithic silica column eluted the analyte with a smaller retention factor (k), lower pressure and satisfactory separation [1]. However, the monolithic silica column has not been widely used for the HPLC or LC/MS analysis of steroids. These ...
متن کاملStability of silica-based, endcapped columns with pH 7 and 11 mobile phases for reversed-phase high-performance liquid chromatography.
The goal of this study was to define practical conditions and limitations of using silica-based, endcapped bonded-phase columns in intermediate and higher pH environments for developing rugged HPLC methods. Bonded-phase degradation in this pH range is a result mainly of silica support dissolution; covalently-bound silane ligands are hydrolyzed very slowly if at all from silica supports at inter...
متن کاملStability Evaluations of an Octadecyl Bonded Phase Based upon Titanium-Grafted Silica
The authors describe the preparation of a C18 bonded phase using silica chemically modified with tetraisopropyl orthotitanate. The product was reacted with ocadecyltrimethoxysilane to yield a reversed phase. The chromatographic performance of this phase was evaluated for the separation of various test mixtures containing polar, nonpolar, and basic compounds, and its stability at pH 9 and 10 was...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Chromatographic Science
سال: 1996
ISSN: 0021-9665,1945-239X
DOI: 10.1093/chromsci/34.7.309